Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

Summary, in English

To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.

• Författare: Q Wanying; T Brodin; Crister Ceberg; Christian Ingvar; Kristina Norrgren; H O Sjogren; Sven-Erik Strand
• Avdelning/ar: Medicinsk strålningsfysik, Lund; Kirurgi, Lund; Medicinsk strålningsfysik, Malmö
• Publiceringsår: 1991
• Språk: Engelska
• Sidor: 379-383
• Publikation/Tidskrift/Serie: Acta Oncologica
• Volym: 30
• Issue: 3
• Länkar: http://dx.doi.org/10.3109/02841869109092390
• Dokumenttyp: Artikel i tidskrift
• Förlag: Taylor & Francis
• Ämne: Cancer and Oncology
• Nyckelord: Monoclonal antibodies; colon carcinoma; rat
• Aktiv: Published
• Forskningsgrupp: Medical Radiation Physics, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1651-226X