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Ceberg

Crister Ceberg

Professor

Ceberg

Rescue Effect Inherited in Colony Formation Assays Affects Radiation Response

Författare

  • Gabriel Adrian
  • Crister Ceberg
  • Ana Carneiro
  • Lars Ekblad

Summary, in English

It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells, surviving fraction increased 1.9-to-4.1-fold after 5-12 Gy irradiation; and for MM576 human melanoma cells, surviving fraction increased 1.9-fold after 5 Gy irradiation. Furthermore, increased surviving fraction was evident after modulated beam irradiation, where irradiated cells could communicate with nonirradiated cells. Media from dense cell culture also increased surviving fraction. The results suggest that traditional colony formation assays comprise unavoidable cellular communication affecting radiation outcome and the shape of the survival curve. We also propose that the increased in-field surviving fraction after modulated beam irradiation is due to the same effect.

Avdelning/ar

  • Tumörmikromiljö
  • Medicinsk strålningsfysik, Lund
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
  • Huvud- och halscancergruppen

Publiceringsår

2018-01

Språk

Engelska

Sidor

44-52

Publikation/Tidskrift/Serie

Radiation Research

Volym

189

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Radiation Research Society

Ämne

  • Cancer and Oncology
  • Radiology, Nuclear Medicine and Medical Imaging
  • Other Physics Topics

Nyckelord

  • Journal Article

Status

Published

Projekt

  • Signaling mediated effects of radiation therapy

Forskningsgrupp

  • Head and Neck Cancer Research Group

ISBN/ISSN/Övrigt

  • ISSN: 0033-7587