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Freddy Ståhlberg

Expert

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(68)Ga-labeled superparamagnetic iron oxide nanoparticles (SPIONs) for multi-modality PET/MR/Cherenkov luminescence imaging of sentinel lymph nodes.

Författare

  • Renata Madru
  • Thuy Tran
  • Johan Axelsson
  • Christian Ingvar
  • Adnan Bibic
  • Freddy Ståhlberg
  • Linda Knutsson
  • Sven-Erik Strand

Summary, in English

The aim of this study was to develop (68)Ga-SPIONs for use as a single contrast agent for dynamic, quantitative and high resolution PET/MR imaging of Sentinel Lymph Node (SLN). In addition (68)Ga enables Cherenkov light emission which can be used for optical guidance during resection of SLN. SPIONs were labeled with (68)Ga in ammonium acetate buffer, pH 5.5. The labeling yield and stability in human serum were determined using instant thin layer chromatography. An amount of 0.07-0.1 mL (~5-10 MBq, 0.13 mg Fe) of (68)Ga-SPIONs was subcutaneously injected in the hind paw of rats. The animals were imaged at 0-3 h and 25 h post injection with PET/CT, 9.4 T MR and CCDbased Cherenkov optical systems. A biodistribution study was performed by dissecting and measuring the radioactivity in lymph nodes, kidneys, spleen, liver and the injection site. The labeling yield was 97.3 ± 0.05% after 15 min and the (68)Ga-SPIONs were stable in human serum. PET, MR and Cherenkov luminescence imaging clearly visualized the SLN. Biodistribution confirmed a high uptake of the (68)Ga-SPIONs within the SLN. We conclude that generator produced (68)Ga can be labeled to SPIONs. Subcutaneously injected (68)Ga-SPIONs can enhance the identification of the SLNs by combining sensitive PET and high resolution MR imaging. Clinically, hybrid PET/MR cameras are already in use and (68)Ga-SPIONs have a great potential as a single-dose, tri-modality agent for diagnostic imaging and potential Cherenkov luminescent guided resection of SLN.

Avdelning/ar

  • Medicinsk strålningsfysik, Lund
  • Lund University Bioimaging Center
  • Kirurgi, Lund
  • MR Physics

Publiceringsår

2014

Språk

Engelska

Sidor

60-69

Publikation/Tidskrift/Serie

American journal of nuclear medicine and molecular imaging

Volym

4

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

e-Century Publishing

Ämne

  • Radiology, Nuclear Medicine and Medical Imaging

Status

Published

Forskningsgrupp

  • MR Physics

ISBN/ISSN/Övrigt

  • ISSN: 2160-8407