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Linda Knutsson

Professor

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99mTc-Labeled Superparamagnetic Iron Oxide Nanoparticles for Multimodality SPECT/MRI of Sentinel Lymph Nodes.

Författare

  • Renata Madru
  • Pontus Kjellman
  • Fredrik Olsson
  • Karin Wingårdh
  • Christian Ingvar
  • Freddy Ståhlberg
  • Johan Olsrud
  • Jimmy Lätt
  • Sarah Fredriksson
  • Linda Knutsson
  • Sven-Erik Strand
Visa allt

Summary, in English

The purpose of this study was to develop multimodality SPECT/MRI contrast agents for sentinel lymph node (SLN) mapping in vivo. METHODS: Nanoparticles with a solid iron oxide core and a polyethylene glycol coating were labeled with (99m)Tc. The labeling efficiency was determined with instant thin-layer chromatography and magnetic separation. The stability of the radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) was verified in both sterile water and human serum at room temperature 6 and 24 h after labeling. Five Wistar rats were injected subcutaneously in the right hind paw with (99m)Tc-SPIONs (25-50 MBq, ∼0.2 mg of Fe) and sacrificed 4 h after injection. Two animals were imaged with SPECT/MRI. All 5 rats were dissected; the lymph nodes, liver, kidneys, spleen, and hind paw containing the injection site were removed and weighed; and activity in the samples was measured. The microdistribution within the lymph nodes was studied with digital autoradiography. RESULTS: The efficiency of labeling of the SPIONs was 99% 6 h after labeling in both water and human serum. The labeling yield was 98% in water and 97% in human serum 24 h after labeling. The SLN could be identified in vivo with SPECT/MRI. The accumulation of (99m)Tc-SPIONs (as the percentage injected dose/g [%ID/g]) in the SLN was 100 %ID/g, whereas in the liver and spleen it was less than 2 %ID/g. Digital autoradiography images revealed a nonhomogeneous distribution of (99m)Tc-SPIONs within the lymph nodes; nanoparticles were found in the cortical, subcapsular, and medullary sinuses. CONCLUSION: This study revealed the feasibility of labeling SPIONs with (99m)Tc. The accumulation of (99m)Tc-SPIONs in lymph nodes after subcutaneous injection in animals, verified by SPECT/MRI, is encouraging for applications in breast cancer and malignant melanoma.

Avdelning/ar

  • MultiPark: Multidisciplinary research focused on Parkinson´s disease
  • eSSENCE: The e-Science Collaboration
  • Medicinsk strålningsfysik, Lund
  • Kirurgi, Lund
  • Diagnostisk radiologi, Lund
  • MR Physics

Publiceringsår

2012

Språk

Engelska

Sidor

459-463

Publikation/Tidskrift/Serie

Journal of Nuclear Medicine

Volym

53

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Society of Nuclear Medicine

Ämne

  • Radiology, Nuclear Medicine and Medical Imaging

Nyckelord

  • Tc-99m
  • lymph nodes
  • SPECT/MRI
  • nanoparticles
  • iron oxide
  • SPIONs

Status

Published

Forskningsgrupp

  • MR Physics

ISBN/ISSN/Övrigt

  • ISSN: 0161-5505