Three-dimensional architecture of human diabetic peripheral nerves revealed by X-ray phase contrast holographic nanotomography

Summary, in English

A deeper knowledge of the architecture of the peripheral nerve with three-dimensional (3D) imaging of the nerve tissue at the sub-cellular scale may contribute to unravel the pathophysiology of neuropathy. Here we demonstrate the feasibility of X-ray phase contrast holographic nanotomography to enable 3D imaging of nerves at high resolution, while covering a relatively large tissue volume. We show various subcomponents of human peripheral nerves in biopsies from patients with type 1 and 2 diabetes and in a healthy subject. Together with well-organized, parallel myelinated nerve fibres we show regenerative clusters with twisted nerve fibres, a sprouted axon from a node of Ranvier and other specific details. A novel 3D construction (with movie created) of a node of Ranvier with end segment of a degenerated axon and sprout of a regenerated one is captured. Many of these architectural elements are not described in the literature. Thus, X-ray phase contrast holographic nanotomography enables identifying specific morphological structures in 3D in peripheral nerve biopsies from a healthy subject and from patients with type 1 and 2 diabetes.

• Författare: Lars B. Dahlin; Kristian R. Rix; Vedrana A. Dahl; Anders B. Dahl; Janus N. Jensen; Peter Cloetens; Alexandra Pacureanu; Simin Mohseni; Niels O.B. Thomsen; Martin Bech
• Avdelning/ar: Handkirurgi, Malmö; Medicinsk strålningsfysik, Lund
• Publiceringsår: 2020
• Språk: Engelska
• Publikation/Tidskrift/Serie: Scientific Reports
• Volym: 10
• Issue: 1
• Länkar: http://dx.doi.org/10.1038/s41598-020-64430-5
• Dokumenttyp: Artikel i tidskrift
• Förlag: Nature Publishing Group
• Ämne: Medical Image Processing; Radiology, Nuclear Medicine and Medical Imaging; Other Physics Topics
• Status: Published
• Forskningsgrupp: Hand Surgery, Malmö
• ISBN/ISSN/Övrigt: ISSN: 2045-2322