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X-ray Phase-contrast imaging

Martin Bech

Universitetslektor

X-ray Phase-contrast imaging

Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging

Författare

  • A. Tapfer
  • Martin Bech
  • I. Zanette
  • P. Symvoulidis
  • S. Stangl
  • G. Multhoff
  • M. Molls
  • V. Ntziachristos
  • F. Pfeiffer

Summary, in English

In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, we investigate cryotome-based planar epi-illumination imaging (cryo-imaging). On the other hand, we examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo-frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X-ray phase-contrast imaging is based on the minute refraction of X-rays inside the specimen and features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To explore the potential of both techniques for studying whole mouse disease models, one mouse specimen was imaged using both techniques. Obtained data are compared visually and quantitatively, specifically with regard to the visibility of fine anatomical details. Internal structure of the mouse specimen is visible in great detail with both techniques and the study shows in particular that soft-tissue contrast is strongly enhanced in the X-ray phase images compared to the attenuation-based images. This identifies phase-contrast micro-CT as a powerful tool for the study of small animal disease models.

Avdelning/ar

  • Medicinsk strålningsfysik, Lund

Publiceringsår

2014

Språk

Engelska

Sidor

24-30

Publikation/Tidskrift/Serie

Journal of Microscopy

Volym

253

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Radiology, Nuclear Medicine and Medical Imaging

Nyckelord

  • Computed tomography
  • planar epi-illumination imaging
  • X-ray phase
  • contrast

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0022-2720