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X-ray Phase-contrast imaging

Martin Bech

Universitetslektor

X-ray Phase-contrast imaging

Radiopaque dyes allow vessel imaging in lung tissue using laboratory phase contrast micro-CT

Författare

  • Till Dreier
  • Gustaf Bernström
  • Sahel Ganji
  • Christian Norvik
  • Karin Tran Lundmark
  • Martin Bech

Redaktör

  • Bert Müller
  • Ge Wang

Summary, in English

Phase contrast micro-CT is a powerful technique allowing imaging of soft tissue at synchrotrons or using lab- oratory sources. The use of contrast agents is a useful approach when imaging vascular structures. However, common x-ray contrast agents typically rely on heavy metals to increase absorption, which may affect the phase contrast and cause artifacts in the reconstructed volumes. Thus, utilizing an agent with lower attenuation similar to soft tissue is clearly advantageous. Here, we evaluated different colored radiopaque solutions (tissue marking dyes) which had been injected into the vascular system of bovine lung samples, prior to embedding in paraffin. Scans were performed using a micro-focus x-tube calibrated to 10 µm spot size at 70 kV and a photon counting detector with a silicon sensor and 75 µm pixels. The resulting volumes have a voxel size of (25 µm)3, limited by the size of the samples, but sufficient to resolve the vascular system. Experiments confirmed that sufficient perfusion of the vessels with the dyes could be achieved, and the different dyes could be clearly discerned in the reconstructed volumes without causing artifacts allowing to clearly identify features in the soft tissue. Further, the findings were confirmed by histology.
Keywords:

Avdelning/ar

  • LTH profilområde: Teknik för hälsa
  • Medicinsk strålningsfysik, Lund
  • Kärlväggsbiologi
  • WCMM- Wallenberg center för molekylär medicinsk forskning
  • X-ray Phase Contrast

Publiceringsår

2022-10-14

Språk

Engelska

Publikation/Tidskrift/Serie

Proceedings of SPIE - The International Society for Optical Engineering

Volym

12242

Dokumenttyp

Konferensbidrag

Förlag

SPIE

Ämne

  • Medical Image Processing

Conference name

Developments in X-Ray Tomography XIV 2022

Conference date

2022-08-22 - 2022-08-24

Conference place

San Diego, United States

Status

Published

Projekt

  • Industrial PhD student

Forskningsgrupp

  • Vessel Wall Biology
  • X-ray Phase Contrast